Everyone has an aim to achieve in his or her life time, what's mine?
I have a few in my mind at the moment, sadly all of them seems far yet I seriously feel I needed to achieve that..Just read through MalysiaKini, Updated myself with some news about fuel price hikes event in Malaysia, having so many unhappy comments, feel sort of unhappy, furious about what and how the government have been treated the people in his country! At the same time, there is some sourness because it is my beloved home country, a country that I have live in for the past 19 years of my life, what does it means? It contains more than 90% of my memory, 90% of my lfietime spent in the same country.but what it have done?
Disappointment after disappointment, when will the gov realise what is going to happen, what is going to be in the future, did they plan? do they ever thought about it? What is about future? U have massive amount of high quality palm oil, but what they have done? nothing except sell it for crap price..why they need to do that? god knows..What I can do? will the idea of biofuel accepted? by a country who are exporting oil? but yet at the same time importing low quality oil? Would they be any biotechnologist in the country to step up and get the idea around the leader? I aint think so..its so sad yet pathetic..
A country with 3 races, with more than hundred or thousands of great food, wide variety until u cant having try all of that in one week, but yet, gov policies of having bumiputeras in local company or gov department has destroyed everything...when the quota would have be gone? Until when we still need to get out of the country to survive? why? its my country, its our country..sad..
Anyway, not reli hope my point being heard or anything, just having it said..not very loud, but at least i sound out..the 'aim'. it would sound perfect if i can serve my country, but do they need me? Will they ever appreciate what we did? Its getting more grey in having a developed country in such a short period of time..not until they are awake...
Biotechnology is the key, at least in near future, biofuel will take over, for the time after petroleum fuel is gone and right before hydrogen fuel is ruling the whole industry, and I hope they can see that..more to be mentioned, the policies, the rules and regulation, its just pathetic after all...
If I have graduated as a Microbiologist, what I can do for my country? There is lots of thing That I can do for the sake of the country but yet would it be appreciate?
Learning to appreciate..live a life that will not regret in later life...and I am still waiting for the calls...
Friday, June 13, 2008
Thursday, May 29, 2008
End of one wave..Another is coming..
Today is the last day of presentation for this semester..just finished Bacteria and Disease presentation and it went well..since Mini proj's presentation, has been having some sort of mental stresses..Sigh...now have Biotech Report and Grant Research Proposal..
Even a pHD student never do GRP before..what a Great practice for me..hope I can go through it..The report is also a hardcore job...reli hope that everything can go thorugh quickly and fly to melbourne and tasmania soon!!!
Anyway, just read Pig head's blog, what a relief she has been safe..but somehow getting bored of her job. Be patient!! I work in supermarket for 1 and half years, in lab for 6months now, everything is sort of the same, I mean in the supermarket..just require your manpower..not much of brain stuff..But lab is diff, always brain...thats y I have headache recently...
Never have a Panda eye, not even when I am studying in TARC or UNSW, but the working time, lab time, assignment have rewarded me with a slight dark ring..omg..I reli dun hope this would affect my health..LIFE LIFE LIFE..
What is the main purpose of getting money, earning money? Is experience worth more than that? What if you have someone or something that u get hold of..like a Family! Would it change your mind..
Even a pHD student never do GRP before..what a Great practice for me..hope I can go through it..The report is also a hardcore job...reli hope that everything can go thorugh quickly and fly to melbourne and tasmania soon!!!
Anyway, just read Pig head's blog, what a relief she has been safe..but somehow getting bored of her job. Be patient!! I work in supermarket for 1 and half years, in lab for 6months now, everything is sort of the same, I mean in the supermarket..just require your manpower..not much of brain stuff..But lab is diff, always brain...thats y I have headache recently...
Never have a Panda eye, not even when I am studying in TARC or UNSW, but the working time, lab time, assignment have rewarded me with a slight dark ring..omg..I reli dun hope this would affect my health..LIFE LIFE LIFE..
What is the main purpose of getting money, earning money? Is experience worth more than that? What if you have someone or something that u get hold of..like a Family! Would it change your mind..
Tuesday, May 20, 2008
Presentation Chain
What is the purposes of presentation? To let people know or understand some concept that they haven't reli look into? Do I need to take it as if they dun know everything? Your audience are all dumb and not understand, so please be concern and clear cut..that What I have been told by one of my lecturer in the past..
So what is my presentation and what is it all about? Summary of the presentation are as below:
1) Environmental Microbiology
Marine bacteria Pseudoalteromonas tunicata is capable of producing broad range of inhibitory compounds including antifungal, anti-larvae, antidiatom, antibacteria and etc. It appears to be one of the major focus of in the finding of bioactive compounds from the marine environment in corporating in biofouling activity. for example, AlpP is a bacteriolytic protein produced by P. tunicata that has antimicrobial properties by disrupting the inner membrane of a bacteria leading to rapid leakage of inner cell component such as iron and others and subsequently cell death is occur. How does it relate to us? It has a high potential in providing environmental friendly solution for marine biofouling problem and increase energy efficiency in shipping industry by prevention of fouling on ship surfaces. It could played a role that has highest impact to human since earth's resources is gradually depleting, providing an alternative to conserve energy resources of the earth.
2) Bacteria and Disease
The role of iron in bacterial pathogenesis is enormous in causing disease. Iorn could acts as a transcriptional regulator for Fur gene by altering the gene expression thus leading to other virulence factors to be transcribed. Iron is one of the major growth factor for bacterial growth, without iron bacterial will not survive nor multiply. Therefore bacteria has acquired several different methods to recruit iron from the environment or from host. example of iron scavenging leads to disease is the production of hemolysin by S. aureus could lyse red blood cell and releasing iron from the hemoglobin. End result is the bacteria could obtained iron while the host would having a severe damage to the system due to extensive cell lysis if the bacteria is multiplying in amount. More...
3) Marine Microbiology Project
A natural bioactive compound, Pyocyanin is produced synergistically by two distinct marine bacterial species. The isolation of marine bacteria from Hawaiian Islands to look for natural product that has antibacterial and antifungal activities. Unusual green phenotype produced leads to the study of antibacterial and antifungal assay using E. coli and S. cerevisieae. Production comes from two bacterial species, Enterobacter and Pseudomonas aeruginosa. Interaction study responsible for the produciton of pyocyanin include inhibition assay, Ratio determinant assay, Small molecule induction assay and Boyden Chamber assay suggesting that a complex mechanism of metabolite exchange between two bacterial species is favorable option. Characterisation of the natural product (pyocyanin) using X-ray structure, 2D-NMR, and more also review the properties of pyocyanin. Moreover, the mode of action of the pyocyanin was tested using trancriptomic assay of yeast. Pyocyanin was found out to be the inducer of oxidative stress, cell wall damage and DNA damage as the most probable mode of action. Result showed that production of natural bioactive compounds could have been related to a bacterial communities rather than pure culture that has been practising in the past.
That's roughly what is all my project about..anyone interested in it could possibly hit a reply and comment..Thanks
Its a good rehearsal though to brainstorming any information that could be left out..but sure of cz its not everything in here...Haha
Alright, time to do stuff!
So what is my presentation and what is it all about? Summary of the presentation are as below:
1) Environmental Microbiology
Marine bacteria Pseudoalteromonas tunicata is capable of producing broad range of inhibitory compounds including antifungal, anti-larvae, antidiatom, antibacteria and etc. It appears to be one of the major focus of in the finding of bioactive compounds from the marine environment in corporating in biofouling activity. for example, AlpP is a bacteriolytic protein produced by P. tunicata that has antimicrobial properties by disrupting the inner membrane of a bacteria leading to rapid leakage of inner cell component such as iron and others and subsequently cell death is occur. How does it relate to us? It has a high potential in providing environmental friendly solution for marine biofouling problem and increase energy efficiency in shipping industry by prevention of fouling on ship surfaces. It could played a role that has highest impact to human since earth's resources is gradually depleting, providing an alternative to conserve energy resources of the earth.
2) Bacteria and Disease
The role of iron in bacterial pathogenesis is enormous in causing disease. Iorn could acts as a transcriptional regulator for Fur gene by altering the gene expression thus leading to other virulence factors to be transcribed. Iron is one of the major growth factor for bacterial growth, without iron bacterial will not survive nor multiply. Therefore bacteria has acquired several different methods to recruit iron from the environment or from host. example of iron scavenging leads to disease is the production of hemolysin by S. aureus could lyse red blood cell and releasing iron from the hemoglobin. End result is the bacteria could obtained iron while the host would having a severe damage to the system due to extensive cell lysis if the bacteria is multiplying in amount. More...
3) Marine Microbiology Project
A natural bioactive compound, Pyocyanin is produced synergistically by two distinct marine bacterial species. The isolation of marine bacteria from Hawaiian Islands to look for natural product that has antibacterial and antifungal activities. Unusual green phenotype produced leads to the study of antibacterial and antifungal assay using E. coli and S. cerevisieae. Production comes from two bacterial species, Enterobacter and Pseudomonas aeruginosa. Interaction study responsible for the produciton of pyocyanin include inhibition assay, Ratio determinant assay, Small molecule induction assay and Boyden Chamber assay suggesting that a complex mechanism of metabolite exchange between two bacterial species is favorable option. Characterisation of the natural product (pyocyanin) using X-ray structure, 2D-NMR, and more also review the properties of pyocyanin. Moreover, the mode of action of the pyocyanin was tested using trancriptomic assay of yeast. Pyocyanin was found out to be the inducer of oxidative stress, cell wall damage and DNA damage as the most probable mode of action. Result showed that production of natural bioactive compounds could have been related to a bacterial communities rather than pure culture that has been practising in the past.
That's roughly what is all my project about..anyone interested in it could possibly hit a reply and comment..Thanks
Its a good rehearsal though to brainstorming any information that could be left out..but sure of cz its not everything in here...Haha
Alright, time to do stuff!
Thursday, May 1, 2008
No Matter - Angel
A song that bring up all the feeling in a headache night..soon after Bacteria and Disease Mid session exam, its been a totally hectic moment where headache started to strike all in a sudden and lasted for a whole day!
However, meeting with Staffan brings good news and have got the full support from him, happy with that.
Went back to Coles to get something, met Mochtar, chat like never seen him for a very very long time.
Remember of a Song - Ming Tian Jian by Cindy Wong reminds me of another person..which is so much more feeling involved in a day.
Its been more than a day, and still I found it sank quite deep into the ocean and would never come up again..All these feeling seems to drive my day..
Time to get back to study!
However, meeting with Staffan brings good news and have got the full support from him, happy with that.
Went back to Coles to get something, met Mochtar, chat like never seen him for a very very long time.
Remember of a Song - Ming Tian Jian by Cindy Wong reminds me of another person..which is so much more feeling involved in a day.
Its been more than a day, and still I found it sank quite deep into the ocean and would never come up again..All these feeling seems to drive my day..
Time to get back to study!
Thursday, April 24, 2008
25 April 2008
A day where its a so called 'holiday' for me! Just finish work from 4-8am, so its free for the rest of the day..up to me for all the rest of the day..
A day where PIG head's Birthday falls on, and its her 21st Birthday! What a big big day! finally BIG enough to vote? haha Hope she will be able to see this today la! HAPPY BIRTHDAY RUI HOONG! feel sorry cant buy her any present..but did sent her a ''voucher'' hahaha..
A day where I have so many different kind of feeling, happy, appreciative, sad, boredom, loneliness, funny, sarcastism, anger,...etc etc I guess the main cause would be email sent by Pig head, a song from Cousin long time ago..just found out when I rearrange my pictures taken recently, all my result's pic, Pics for Beach Volleyball, second round is just around the corner and so sad that I will definitely miss it..look at all those clips, analysing my football skills looking at those video clips of mine when we were in Sunway in Feb...
A day where I look at all those memorable video clips
A day where I will have to go back to lab..which is usual..
A day where I would play Facebook, been daily activities recently..haha
A day where I can sleep later than 9.30pm at night..
A day where I decided to write this blog!
Alright, better get going..lab time, revision is on the way since mid session exam is fallling on next week and unfortunately 3 in a roll..Lucky Me..
See ya..Again, Happy Birthday to my Pig Head Sis!
A day where PIG head's Birthday falls on, and its her 21st Birthday! What a big big day! finally BIG enough to vote? haha Hope she will be able to see this today la! HAPPY BIRTHDAY RUI HOONG! feel sorry cant buy her any present..but did sent her a ''voucher'' hahaha..
A day where I have so many different kind of feeling, happy, appreciative, sad, boredom, loneliness, funny, sarcastism, anger,...etc etc I guess the main cause would be email sent by Pig head, a song from Cousin long time ago..just found out when I rearrange my pictures taken recently, all my result's pic, Pics for Beach Volleyball, second round is just around the corner and so sad that I will definitely miss it..look at all those clips, analysing my football skills looking at those video clips of mine when we were in Sunway in Feb...
A day where I look at all those memorable video clips
A day where I will have to go back to lab..which is usual..
A day where I would play Facebook, been daily activities recently..haha
A day where I can sleep later than 9.30pm at night..
A day where I decided to write this blog!
Alright, better get going..lab time, revision is on the way since mid session exam is fallling on next week and unfortunately 3 in a roll..Lucky Me..
See ya..Again, Happy Birthday to my Pig Head Sis!
Friday, April 18, 2008
Finally, its time to take a deep Breath!!
Its been a long long time where I could have a afternoon nap for 2hrs..lol
Have been working overtime for the last few day, sleep only 5hrs a day, work 4-8am in the morning in supermarket, then head back to the lab to carry out experiment, and attend classes in sort of tiredness..
Work in Coles is not too hard, starting not to care too much, people takign advantage when u work too hard, take it for granted..and to work by myself until 8am where there should be another person coming in at 7am..so so so freaking crazy..not suppose to be like tat...How can you just leave me to work by myself..??
Study, busy time, mid session, assignment, just submitted Poster Abstract for Envi. Micro on Wednesday and literature search for BABS3301 today, more is coming next week where Bacteria & Disease essay on Monday, Intro + Method for Env. Micro Major Project on Wednesday, Biotech Primer Design on Thursday..Omg..so scary when I think about it..
Lab work, hard hard hard..my bacteria doesn't seems to mutate. lasy bastard, rather die than mutate..want a spontaneous mutant of bacteria resistant to Streptomycin 200ug/ml! Once I get mutant have to go into transposon mutagenesis straight away...require to screen at least 1000+ mutant for statistical significant result..worrying not able to finish the exp in time..dun wana disappoint Staffan since he gave me such an opportunity..Work Hard Work Hard!
Back to work, chao..
Have been working overtime for the last few day, sleep only 5hrs a day, work 4-8am in the morning in supermarket, then head back to the lab to carry out experiment, and attend classes in sort of tiredness..
Work in Coles is not too hard, starting not to care too much, people takign advantage when u work too hard, take it for granted..and to work by myself until 8am where there should be another person coming in at 7am..so so so freaking crazy..not suppose to be like tat...How can you just leave me to work by myself..??
Study, busy time, mid session, assignment, just submitted Poster Abstract for Envi. Micro on Wednesday and literature search for BABS3301 today, more is coming next week where Bacteria & Disease essay on Monday, Intro + Method for Env. Micro Major Project on Wednesday, Biotech Primer Design on Thursday..Omg..so scary when I think about it..
Lab work, hard hard hard..my bacteria doesn't seems to mutate. lasy bastard, rather die than mutate..want a spontaneous mutant of bacteria resistant to Streptomycin 200ug/ml! Once I get mutant have to go into transposon mutagenesis straight away...require to screen at least 1000+ mutant for statistical significant result..worrying not able to finish the exp in time..dun wana disappoint Staffan since he gave me such an opportunity..Work Hard Work Hard!
Back to work, chao..
Monday, April 7, 2008
Excited!!!
Well..a great great news for me yesterday!
Just got the offer letter from UNSW Scholarship department regarding the offer of UNSW Honours Year Scholarship..This is a freaking big news which I will never expected at anytime..declare Holiday if I can..hahaha
Anyway, just trying so hard to share the joy with everyone that I loved and care for..Things getting better, smoother, life still goes on!! Go Go Go Pigbrain..Thats another day and time to start doing transposon mutagenesis work from today onwards..Another challenge that I would face..
I am so glad that I could have that in my life..
Well, UNSW Div 2 Volleyball team just won a Bronze in Illawarra State Cup without me..But I won the Volleyball Championship for MSO UNSW in Malaysian Student Inter-Uni Game! Its fun though..One or another..thats life!
Time to keep going!
Just got the offer letter from UNSW Scholarship department regarding the offer of UNSW Honours Year Scholarship..This is a freaking big news which I will never expected at anytime..declare Holiday if I can..hahaha
Anyway, just trying so hard to share the joy with everyone that I loved and care for..Things getting better, smoother, life still goes on!! Go Go Go Pigbrain..Thats another day and time to start doing transposon mutagenesis work from today onwards..Another challenge that I would face..
I am so glad that I could have that in my life..
Well, UNSW Div 2 Volleyball team just won a Bronze in Illawarra State Cup without me..But I won the Volleyball Championship for MSO UNSW in Malaysian Student Inter-Uni Game! Its fun though..One or another..thats life!
Time to keep going!
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