Presentation Chain

What is the purposes of presentation? To let people know or understand some concept that they haven't reli look into? Do I need to take it as if they dun know everything? Your audience are all dumb and not understand, so please be concern and clear cut..that What I have been told by one of my lecturer in the past..

So what is my presentation and what is it all about? Summary of the presentation are as below:

1) Environmental Microbiology
Marine bacteria Pseudoalteromonas tunicata is capable of producing broad range of inhibitory compounds including antifungal, anti-larvae, antidiatom, antibacteria and etc. It appears to be one of the major focus of in the finding of bioactive compounds from the marine environment in corporating in biofouling activity. for example, AlpP is a bacteriolytic protein produced by P. tunicata that has antimicrobial properties by disrupting the inner membrane of a bacteria leading to rapid leakage of inner cell component such as iron and others and subsequently cell death is occur. How does it relate to us? It has a high potential in providing environmental friendly solution for marine biofouling problem and increase energy efficiency in shipping industry by prevention of fouling on ship surfaces. It could played a role that has highest impact to human since earth's resources is gradually depleting, providing an alternative to conserve energy resources of the earth.

2) Bacteria and Disease
The role of iron in bacterial pathogenesis is enormous in causing disease. Iorn could acts as a transcriptional regulator for Fur gene by altering the gene expression thus leading to other virulence factors to be transcribed. Iron is one of the major growth factor for bacterial growth, without iron bacterial will not survive nor multiply. Therefore bacteria has acquired several different methods to recruit iron from the environment or from host. example of iron scavenging leads to disease is the production of hemolysin by S. aureus could lyse red blood cell and releasing iron from the hemoglobin. End result is the bacteria could obtained iron while the host would having a severe damage to the system due to extensive cell lysis if the bacteria is multiplying in amount. More...

3) Marine Microbiology Project
A natural bioactive compound, Pyocyanin is produced synergistically by two distinct marine bacterial species. The isolation of marine bacteria from Hawaiian Islands to look for natural product that has antibacterial and antifungal activities. Unusual green phenotype produced leads to the study of antibacterial and antifungal assay using E. coli and S. cerevisieae. Production comes from two bacterial species, Enterobacter and Pseudomonas aeruginosa. Interaction study responsible for the produciton of pyocyanin include inhibition assay, Ratio determinant assay, Small molecule induction assay and Boyden Chamber assay suggesting that a complex mechanism of metabolite exchange between two bacterial species is favorable option. Characterisation of the natural product (pyocyanin) using X-ray structure, 2D-NMR, and more also review the properties of pyocyanin. Moreover, the mode of action of the pyocyanin was tested using trancriptomic assay of yeast. Pyocyanin was found out to be the inducer of oxidative stress, cell wall damage and DNA damage as the most probable mode of action. Result showed that production of natural bioactive compounds could have been related to a bacterial communities rather than pure culture that has been practising in the past.

That's roughly what is all my project about..anyone interested in it could possibly hit a reply and comment..Thanks

Its a good rehearsal though to brainstorming any information that could be left out..but sure of cz its not everything in here...Haha

Alright, time to do stuff!